| Group | Club/Team Name | Team ID | Head Coach |
| 443-15 | Apex Soccer Academy Apex 9UB Storm |
0443-01AB16-0015 | Caden Maher |
| 402-1 | Bondurant Soccer Club BSC U9 Academy Blue |
0402-01AB16-0001 | Mark Feld |
| 429-11 | Dallas Center Grimes Soccer Club DCG Academy U9 Boys Red |
0429-01AB16-0011 | Wes Dawson |
| 441-50 | Des Moines Soccer Club DMSC 2016 Boys Blue |
0441-01AB16-0050 | Matt Lamale |
| 441-30 | Des Moines Soccer Club DMSC 2016 Boys Red |
0441-01AB16-0030 | Matt Lamale |
| 441-51 | Des Moines Soccer Club DMSC 2016 Boys White |
0441-01AB16-0051 | Matt Lamale |
| 445-2 | Genesis Football Club Genesis FC 2016/2017 Boys |
0445-01AB16-0002 | Sam Gabriel |
| 418-21 | J-Hawk Soccer Club Iowa United FC Academy 9UB Red |
0418-01AB16-0021 | Ames Cross |
| 418-22 | J-Hawk Soccer Club Iowa United FC Academy 9UB White |
0418-01AB16-0022 | Scott Motter |
| 430-25 | Indianola Soccer Tribe IST Academy U9 Boys |
0430-01AB16-0025 | Dillon Phillips |
| 421-46 | Pleasant Hill Soccer Club PHSC BU9 Academy Black |
0421-01AB15-0046 | Jorge Ibarra |
| 421-47 | Pleasant Hill Soccer Club PHSC BU9 Academy Orange |
0421-01AB16-0047 | Herbie Underwood |
| 425-31 | Spring Creek FC SCFC BOYS ACADEMY - U9B |
0425-01AB16-0031 | Mefi Hernandez |
| 422-25 | United Futbol Academy UFA DSM 2016B A2 |
0422-01AB16-0025 | Dennis Diaz |
| 422-41 | United Futbol Academy UFA DSM 2016B A3 |
0422-01AB16-0041 | n/a |
| 422-40 | United Futbol Academy UFA DSM 2017B |
0422-01AB16-0040 | Juan Martinez |
| 301-1 | Prairie Soccer Club (PSC) Iowa Prairie Soccer Club (PSC) - 9U Boys Academy |
0301-01AB16-0001 | n/a |