| Group | Club/Team Name | Team ID | Head Coach |
| 401-6 | Ballard Soccer Club 12U Ballard Coed |
0401-01RB13-0006 | Brett Franzen |
| 426-14 | Adel DeSoto Soccer Club ADMSC B12A |
0426-01RB13-0014 | Nathan Beck |
| 427-5 | Boone Soccer Club Boone 12U Co-ed |
0427-01RB13-0005 | Natasha Moyer |
| 415-16 | Collins-Maxwell Rec Soccer club Collins-Maxwell U12 |
0415-01RB13-0016 | Nancy Shryock |
| 448-1 | Christian Soccer Academy CSA U12 |
0448-01RB13-0001 | Michael David |
| 428-6 | Carlisle Soccer Club CSC U12A Boys |
0428-01RB13-0006 | Cody Kooi |
| 428-7 | Carlisle Soccer Club CSC U12B Boys |
0428-01RB13-0007 | Raul Saucedo |
| 429-24 | Dallas Center Grimes Soccer Club DCG B12A |
0429-01RB13-0024 | Stephanie Maher |
| 441-40 | Des Moines Soccer Club DMSC 12U Boys Rec 1 |
0441-01RB13-0040 | Ted Nellesen |
| 441-41 | Des Moines Soccer Club DMSC 12U Boys Rec 2 |
0441-01RB13-0041 | Nda Yao |
| 427-7 | Boone Soccer Club GC 12U Boys |
0427-01RB13-0007 | Chad Schwander |
| 425-28 | Spring Creek FC Grinnell Mid Iowa - B2013 |
0425-01RB13-0028 | Daniel Rodrigues |
| 430-29 | Indianola Soccer Tribe Indianola Soccer Tribe B12A |
0430-01RB13-0029 | Nigel Worby |
| 430-30 | Indianola Soccer Tribe Indianola Soccer Tribe B12B |
0430-01RB13-0030 | Chad Bottorff |
| 430-31 | Indianola Soccer Tribe Indianola Soccer Tribe B12C |
0430-01RB13-0031 | Mike Tiano |
| 431-16 | Iowa Rush South Iowa Rush South Fall 2024 B12 |
0431-01RB13-0016 | Matt Rauch |
| 432-6 | Knoxville Soccer Club Knoxville Socc - B2013 |
0432-01RB13-0006 | Tanner Peterson |
| 419-15 | Marshalltown Area Soccer Club Marshalltown Area Soccer Club - B12U |
0419-01RB13-0015 | Robert Hazen |
| 419-11 | Marshalltown Area Soccer Club Marshalltown Area Soccer Club - Hustlers |
0419-01RB13-0011 | Jeff McKibbin |
| 419-14 | Marshalltown Area Soccer Club Marshalltown Area Soccer Club - Storm |
0419-01RB13-0014 | Ben Ahrens |
| 408-9 | Nevada Soccer Club N12A |
0408-01RB13-0009 | Ashley Whitehead |
| 409-12 | Newton Area Soccer Association NASA B12A |
0409-01RB13-0012 | Cortney Henry |
| 409-11 | Newton Area Soccer Association NASA B12B |
0409-01RB13-0011 | Ryan Riggs |
| 435-3 | Northwest Soccer Club Northwest Socc - B2013 C12A |
0435-01RB13-0003 | Mark Hollister |
| 420-18 | North Polk United Soccer Club NPU B12A |
0420-01RB13-0018 | Joe Ruddy |
| 420-19 | North Polk United Soccer Club NPU B12B |
0420-01RB13-0019 | Michael Kline |
| 434-3 | Norwalk Soccer Club NSC 12U Boys |
0434-01RB13-0003 | Edward Rhodes |
| 410-6 | Panorama Soccer Club Panorama Soccer Club - B2013 12/11U |
0410-01RB13-0006 | Paul Kautzky |
| 411-3 | Perry Soccer Club Perry Soccer - B2013 U12 Blue |
0411-01RB13-0003 | n/a |
| 425-16 | Spring Creek FC Spring Creek FC - 2013 Black |
0425-01RB13-0034 | Timothy Stoll |
| 425-17 | Spring Creek FC Spring Creek FC - 2013 Gray |
0425-01RB13-0035 | Alexander Winkels |
| 422-30 | United Futbol Academy UFA DSM 2012/14Coed CLASSIC |
0422-01RB12-0030 | Jose Alvarez |
| 413-7 | Van Meter Youth Soccer Club Van Meter U11/12 A Boys |
0413-01RB13-0007 | Nicholas Harrison |
| 413-8 | Van Meter Youth Soccer Club Van Meter U11/12 B Boys |
0413-01RB13-0008 | Brandy Christianson |
| 413-9 | Van Meter Youth Soccer Club Van Meter U11/12 C Boys |
0413-01RB13-0009 | Ryan Nixon |
| 423-5 | WCV United Soccer Club WCV United U12-1 Co-Ed |
0423-01RB13-0005 | James Morgan |
| 423-6 | WCV United Soccer Club WCV United U12-2 Co-Ed |
0423-01RB13-0006 | Erin McColloch |
| 437-7 | Winterset Soccer Club Winterset Socc - B2013 |
0437-01RB12-0007 | Brett Hanna |
| 444-2 | Woodward Granger Soccer Club Woodward Granger U12 Boys |
0444-01RB13-0002 | Pat Conway |
| 407-3 | Madrid Soccer Association MSA U12 Coed |
0407-01RB13-0003 | Nathan Boes |