| Group | Club/Team Name | Team ID | Head Coach |
| 443-3 | Apex Soccer Academy Apex 12UB |
0443-01SB13-0003 | Jonathan Jacobson |
| 205-4 | Bettendorf Soccer Association BSA 2013 Boys White |
0205-01SB13-0004 | Joseph Stehn |
| IO03-1 | ISA Independents Chaos United |
IO03-01SB13-0001 | Jamie Cornelius |
| 429-3 | Dallas Center Grimes Soccer Club DCG Boys U12 Select |
0429-01SB13-0003 | Curtis Kampman |
| 441-6 | Des Moines Soccer Club DMSC 2013 Boys Blue |
0441-01SB13-0006 | Aidan Martindale |
| 441-5 | Des Moines Soccer Club DMSC 2013 Boys Red |
0441-01SB13-0005 | Aidan Martindale |
| 445-4 | Genesis Football Club Genesis FC - Beige |
0445-01SB13-0004 | Jonathan Williams |
| 431-3 | Iowa Rush South Iowa Rush South 12U/13U Boys |
0431-01SB12-0003 | Tracie McCulla |
| 442-3 | Iowa Storm Soccer Club Iowa Storm U12B |
0442-01SB13-0003 | Herbie Underwood |
| 418-7 | J-Hawk Soccer Club Iowa United FC 12UB Navy |
0418-01SB13-0007 | Jess Richey |
| 418-8 | J-Hawk Soccer Club Iowa United FC 12UB White |
0418-01SB13-0008 | Matt Lundquist |
| 419-2 | Marshalltown Area Soccer Club Marshalltown Area Soccer Club - Leon |
0419-01SB12-0002 | Santos Martinez |
| 419-3 | Marshalltown Area Soccer Club Marshalltown Area Soccer Club - Little Bobcats |
0419-01SB12-0003 | Jose Gomez |
| 408-1 | Nevada Soccer Club Nevada N12 ISL |
0408-01RB13-0001 | Justin Evans |
| 420-2 | North Polk United Soccer Club NPU U12B |
0420-01SB13-0002 | Nicholas Wilkins |
| 209-5 | Southeast Soccer Academy SESA 2013 B |
0209-01SB13-0005 | Matt Hall |
| 425-3 | Spring Creek FC Spring Creek FC - B2013 |
0425-01SB13-0003 | Semir Ibisevic |
| 422-27 | United Futbol Academy UFA DSM 2013B ELITE |
0422-01SB13-0027 | Fernando Rodriguez Cruz |
| 422-39 | United Futbol Academy UFA DSM 2013B SELECT |
0422-01SB13-0039 | Alex Koons |
| 449-5 | Rozeboom Futbol Academy Rozeboom Futbol Academy (RFA) 2014B |
0449-01SB14-0005 | Lance Rozeboom |
| 441-1 | Des Moines Soccer Club DMSC 2014 Boys Red |
0441-01SB14-0001 | Matt Lamale |