| Group | Club/Team Name | Team ID | Head Coach |
| 426-9 | Adel DeSoto Soccer Club ADMSC B09A |
0426-01RB16-0009 | Karie Livingston |
| 426-10 | Adel DeSoto Soccer Club ADMSC B09B |
0426-01RB16-0010 | Thomas Harmsen |
| 402-5 | Bondurant Soccer Club BSC U9 Blue |
0402-01RB16-0005 | Timothy Hartmann |
| 402-6 | Bondurant Soccer Club BSC U9 White |
0402-01RB16-0006 | n/a |
| 428-3 | Carlisle Soccer Club CSC U9-Boys |
0428-01RB15-0003 | Apisath Saenvong |
| 429-25 | Dallas Center Grimes Soccer Club DCG B9/10A |
0429-01RB15-0025 | Analeigh Sash |
| 441-54 | Des Moines Soccer Club DMSC 9U Boys Rec 2 |
0441-01RB16-0054 | Emmanuel Contreras-Ramos |
| 441-53 | Des Moines Soccer Club DMSC 9U Boys Rec 1 |
0441-01RB16-0053 | Dana Hall |
| 416-1 | Dowling Soccer Club Dowling U9 Coed |
0416-01RB16-0001 | Heath Weiland |
| 430-45 | Indianola Soccer Tribe Indianola Soccer Tribe B9A |
0430-01RB16-0045 | Clark Ridlen |
| 430-46 | Indianola Soccer Tribe Indianola Soccer Tribe B9B |
0430-01RB16-0046 | Nathan Seberg |
| 431-19 | Iowa Rush South Iowa Rush South Fall 2024 B9 |
0431-01RB16-0019 | Chris Allen |
| 418-32 | J-Hawk Soccer Club J-Hawk SC 9UB Red |
0418-01RB16-0032 | Lee Johnson |
| 418-25 | J-Hawk Soccer Club J-Hawk SC 9UB White |
0418-01RB16-0025 | Audra Ramsey |
| 432-9 | Knoxville Soccer Club Knoxville Socc - B2016 |
0432-01RB16-0009 | Kyle Shilling |
| 409-4 | Newton Area Soccer Association NASA B9A |
0409-01RB16-0004 | Jenny Jensen |
| 409-5 | Newton Area Soccer Association NASA B9B |
0409-01RB16-0005 | Carlos Flores |
| 420-24 | North Polk United Soccer Club NPU B9A |
0420-01RB16-0024 | Michael Jones |
| 420-23 | North Polk United Soccer Club NPU B9B |
0420-01RB16-0023 | Gary Meier |
| 420-22 | North Polk United Soccer Club NPU B9C |
0420-01RB16-0022 | Brian Hunt |
| 410-3 | Panorama Soccer Club Panorama Soccer Club - B2016 9U |
0410-01RB16-0003 | Jason Grossman |
| 421-15 | Pleasant Hill Soccer Club PHSC BU9A |
0421-01RB16-0037 | Enrique Garcini |
| 421-06 | Pleasant Hill Soccer Club PHSC BU9B |
0421-01RB16-0038 | Michael Lauters |
| 425-25 | Spring Creek FC Spring Creek FC - B2016 |
0425-01RB16-0025 | Ahmed Ali |
| 437-10 | Winterset Soccer Club Winterset Socc - B2016 |
0437-01RB16-0010 | Erica Smothers |
| 444-9 | Woodward Granger Soccer Club Woodward Granger U9 Boys |
0444-01RB16-0009 | Matthew Jansa |